Spectrophotometric determination of diosgenin in Dioscorea composita following thin-layer chromatography.

نویسندگان

  • G L Sánchez
  • J C Medina Acevedo
  • R R Soto
چکیده

A rapid and accurate method is described for the determination of diosgenin in Dioscorea conzposita roots. After acid hydrolysis and solvent extraction of the root, pure diosgenin is isolated by thin-layer chromatography, treated with sulphuric acid methanol, and the resultant chromophore is measured by spectrophotometry. Similarly, gas liquid chromatography is used to evaluate statistically the accuracy of the method.

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عنوان ژورنال:
  • The Analyst

دوره 97 161  شماره 

صفحات  -

تاریخ انتشار 1972